Journal: Cancer cell

Article Title: Lineage tracing reveals clonal progenitors and long-term persistence of tumor-specific T cells during immune checkpoint blockade

doi: 10.1016/j.ccell.2023.03.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: U-Load Dextramer ® MHC I Kit (HLA-A*01:01, A*02:01, A*03:01, C*07:01) , Immudex , Custom Orders.

Techniques: Blocking Assay, Recombinant, Cell Isolation, Control, Activation Assay, Staining, Sequencing, Software

Journal: Neural Regeneration Research

Article Title: Stress increases MHC-I expression in dopaminergic neurons and induces autoimmune activation in Parkinson's disease

doi: 10.4103/1673-5374.313057

Figure Lengend Snippet: Expression of MHC-I in dopaminergic neurons is increased in Parkinson's disease models. (A) MHC-I expression in SH-SY5Y cells after being incubated with MPP + (0.1 mmol/mL) and INF-γ (100 ng/mL) for 24 hours. (B) MHC-I expression in SH-SY5Y cells after different concentrations of MPP + treatment for 24 hours. (C) MHC-I expression in SH-SY5Y cells after different incubation times with MPP + . (D) Fluorescent images showing MHC-I (green) expression in SH-SY5Y cells. Scale bar: 20 μm. (E) Confocal immunofluorescent labeling of TH (green) and MHC-I (red) in the substantia nigra of mice in the control group, 1-day-after-injection group, and 7-days-after-injection group. Scale bar: 50 μm. (F, G) Numbers of TH + neurons in the substantia nigra of mice and the proportion of TH + neurons with MHC-I expression. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). * P < 0.05, ** P < 0.01, *** P < 0.001. INF-γ: Interferon-γ; MHC-I: major histocompatibility complex class I; MPP + : 1-methyl-4-phenylpyridinium; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.

Article Snippet: To induce the MHC-I knockdown model (Ye et al., 2018), we used a mouse MHC-I knockdown adenovirus designed by GenePharma Co. (Shanghai, China).

Techniques: Expressing, Incubation, Labeling, Injection

Journal: Neural Regeneration Research

Article Title: Stress increases MHC-I expression in dopaminergic neurons and induces autoimmune activation in Parkinson's disease

doi: 10.4103/1673-5374.313057

Figure Lengend Snippet: Expression of MHC-I causes immune attacks in the substantia nigra of Parkinson's disease model mice. (A) Immunofluorescence triple staining for TH (red), MHC-I (green), and CD8 + T cells (pink) in the substantia nigra of the mouse midbrain in the control group, 1-day-after-injection group, and 7-days-after-injection group. Scale bar: 50 μm. (B) Numbers of CD8 + T cells per mm 2 . (C) Representative immunofluorescence double staining for the nucleus (DAPI, blue) and TH (red) in the substantia nigra region. Scale bar: 2 mm. (D) Numbers of TH + neurons in the substantia nigra of mice. (E) Immunofluorescence triple staining for TH (red), MHC-I (green), and CD8 + T cells (pink) in the MPTP + Ad-MHC-1-shRNA group. The red arrow indicates the side that was injected with Ad-MHC-1-shRNA, while the yellow arrow indicates the side that was injected with Ad-NC. Scale bar: 50 μm. (F, G) Numbers of CD8 + T cells and MHC-1-positive neurons per mm 2 . * P < 0.05, ** P < 0.01, *** P < 0.001. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). DAPI: 4′,6-Diamidino-2-phenylindole; MHC-I: major histocompatibility complex class I; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; NC: negative control; TH: tyrosine hydroxylase.

Article Snippet: To induce the MHC-I knockdown model (Ye et al., 2018), we used a mouse MHC-I knockdown adenovirus designed by GenePharma Co. (Shanghai, China).

Techniques: Expressing, Immunofluorescence, Staining, Injection, Double Staining, shRNA, Negative Control

Journal: Neural Regeneration Research

Article Title: Stress increases MHC-I expression in dopaminergic neurons and induces autoimmune activation in Parkinson's disease

doi: 10.4103/1673-5374.313057

Figure Lengend Snippet: PINK1 is related to MHC-I expression in SH-SY5Y cells. (A) Flow cytometric analysis of MHC-I expression in neuron membranes. (B) Quantification of the flow cytometric analysis results. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). * P < 0.05. (C) Western blot assay of PINK1 expression in SH-SY5Y cells that had been transfected with control or PINK1 siRNA. (D) Fluorescence images showing the expression of MHC-1 (green) in SH-SY5Y cells. Scale bar: 5 μm. The arrow indicates MHC-I protein presenting on the cell surface. MHC-I: Major histocompatibility complex class I; MPP + : 1-methyl-4-phenylpyridinium; PINK1: PTEN-induced kinase 1.

Article Snippet: To induce the MHC-I knockdown model (Ye et al., 2018), we used a mouse MHC-I knockdown adenovirus designed by GenePharma Co. (Shanghai, China).

Techniques: Expressing, Western Blot, Transfection, Fluorescence